AviPro Precise Lyophilisate for Use in Drinking Water

from the age of 7 days onwards.
The determination of the vaccination date is depending on a number of factors
including status of maternal antibodies, type of bird, infection pressure, housing and
management conditions.
Maternally derived antibodies (MDA) potentially interfere with the take of live IBD
vaccines. The optimum age for vaccination therefore depends on both, the level of
MDA against IBD in the flock and the capability of the vaccine to take in face of these
MDA (“break-through-titre”). A high homogeneity of the flock MDA levels is important
to define the timing of vaccination and guarantees the better take of the vaccine. To
predict the age, when MDA have sufficiently decreased to allow effective vaccination,
it is advised to test serum samples of at least 24 chicks by serology and apply the
“Deventer Formula” for intermediate vaccines. For chicks from fully vaccinated or
field virus infected breeders this may be at 14 days or more. Serologically negative
birds may be vaccinated from day 7.
A further vaccination 7 days after the first may be necessary particularly in flocks
where antibody levels vary widely between birds (i.e. CV greater than 30 %), or stock
originates from different sources.
Broilers:
- with no maternal antibodies – from 7 days of age
- with maternal antibodies – from 14 days of age
Layers/Breeders:
- with no maternal antibodies – from 7 days of age
- with maternal antibodies – from 3 - 4 weeks of age
Dosage and administration:
Drinking water application:
- Determine the number of vaccine doses and amount of water (see below) required.
Do not split large vials to vaccinate more than 1 house or drinking system, as this
may lead to mixing errors.
- Make sure that all conduit pipes, tubing, troughs, drinkers etc are thoroughly clean
and free of any trace of disinfectants, detergents etc.
- Ensure that the drinking water is cold, clean, and free from detergents and
disinfectants to ensure the viability of the vaccine. Use only fresh water preferably
non-chlorinated and free from metal-ions. Low-fat skimmed milk powder (i.e. < 1 %
fat) may be added to the water (2 – 4 grams per litre) or skimmed milk (20 – 40 ml
per litre of water) to improve the water quality and to increase the stability of the
virus. This, however, has to be done 10 minutes prior to reconstitution of the vaccine.
- Open the vaccine ampoule under water and reconstitute contents thoroughly. Care
should be taken to empty the ampoule and its top completely by rinsing them in
water.
- Allow water to be consumed so that levels in drinkers are minimal before vaccine is
applied. All tubing should be emptied of plain water, so that the drinkers contain only
vaccine water. If water is still present, drain lines before applying vaccine.
- Apply vaccine over (up to) 2 hours, ensuring that all birds drink during this time.
Birds drinking behaviour varies, it may be necessary to withhold water on some sites
prior to vaccination in order to ensure that all birds drink during the vaccination
period.
- The aim is to give every bird one dose of vaccine.
- Ideally vaccine should be administered in the volume of water consumed by the
birds in up to 2 hours. As a general rule, apply reconstituted vaccine to cold and fresh
water at the rate of 1,000 doses of vaccine to 1 litre of water per day of age for 1,000
chickens, e.g. 10 litres would be needed for 1,000, 10 days old chickens. Under hot
climates or with heavy breeds this amount may have to be increased up to a
maximum of 40 litres per 1000 birds. If in doubt, measure water intake the day before
administering vaccine.
- Administer the reconstituted vaccine to birds immediately. Make sure that birds do
not have access to unmedicated water during vaccination.
- The contents of opened bottles should be used all at once.
- Only the volume of vaccine should be prepared which can be applied within 2
hours.