AviPro IBD Xtreme Lyophilisate for Use in Drinking Water for Chickens

Vaccination programme:
The determination of the vaccination date depends on a number of factors including status
of maternal antibodies, type of bird, infection pressure, housing and management
conditions.
AviPro IBD Xtreme can break through a maternal ELISA-antibody level of 636.
Homogenous levels of MDA levels facilitate a more accurate timing of the vaccination.
To predict the age, when MDA have sufficiently decreased to allow effective vaccination it
is advised, to test serum samples of at least 20 chicks by serology and apply the
“Deventer Formula” for intermediate plus vaccines.
According to this formula the optimum age of vaccination is as follows:
1. Decide what percentage of the flock shall be representing and remove the highest
titres that are excluded (e.g. 75 % of the flock; remove the highest 25 % of the titres).
2. Calculate the mean ELISA antibody titre of these birds.
3. Vaccination age = {(log2 titre bird % - log2 breakthrough) x t_} + age at sampling
+ correction 0-4
(Bird % = ELISA titre of the bird representing a certain percentage of the flock
t_ = half-life time (ELISA) of the antibodies in the type of chickens being sampled
Age sampling = age of the birds at sampling
Correction 0-4 = extra days when the sampling was done at 0 to 4 days of age)
Birds should be at least 7 days of age at vaccination. The optimum age for vaccination
may be calculated using the level of maternal antibody in the chicks at day old (“Deventer
Formula”), but normally lies in the range 12-21 days. Additional information on application
and disease control is available from Elanco Animal Health.
Ensure that the drinking water is cold, clean, non-chlorinated and free from detergents,
disinfectants and metal ions.
• Remove sealing cap and stopper from vaccine container.
• Suspend the vaccine in the corresponding amount of water and mix carefully.
• Prepare only the amount of vaccine that can be consumed within 2 hours.
• The vaccine is ready for use.
Drinking water application:
• Determine the number of vaccine doses and amount of water (see below) required.
Do not split large vials to vaccinate more than 1 house or drinking system, as this
may lead to mixing errors.
• Make sure that all conduit pipes, tubing, troughs, drinkers etc. are thoroughly clean
and free of any trace of disinfectants, detergents etc.
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• Use only cold and fresh water preferably non-chlorinated and free from metal-ions.
Low-fat skimmed milk powder (i.e. < 1 % fat) may be added to the water (2-4 grams
per litre) or skimmed milk (20-40 ml per litre of water) to improve the water quality
and to increase the stability of the virus. This however, has to be done at least 10
minutes prior to reconstitution of the vaccine.
• Open the vaccine ampoule under water and reconstitute contents thoroughly. Care
should be taken to empty the ampoule and its top completely by rinsing them in
water.
• Allow water to be consumed so that levels in drinkers are minimal before vaccine is
applied. All tubing should be emptied of plain water, so that the drinkers contain only
vaccine water. If water is still present, drain lines before applying vaccine.
• Apply vaccine over (up to) 2 hours, ensuring that all birds drink during this time. Birds
drinking behaviour varies, it may be necessary to withhold water on some sites prior
to vaccination in order to ensure that all birds drink during the vaccination period.
• Ideally vaccine should be administered in the volume of water consumed by the birds
in up to 2 hours. As a general rule, apply reconstituted vaccine to cold and fresh
water at the rate of 1,000 doses of vaccine to 1 litre of water per day of age for 1,000
chickens, e.g. 10 litres would be needed for 1,000, 10 day old chickens. Under hot
climates or with heavy breeds this amount may have to be increased up to a
maximum of 40 litres per 1,000 birds. If in doubt, measure water intake the day
before administering vaccine.
– Administer the reconstituted vaccine to birds immediately.
– Make sure that birds do not have access to unmedicated water during vaccination.