Poulvac MD Vac Lyophilisate and Diluent for Suspension for Injection

One dose of 0.2 ml per chicken.
The vaccine is to be administered either by intramuscular injection in
the thigh or by subcutaneous injection in the neck to one-day-old
chickens.
Reconstitute each 1,000 doses of Poulvac MD-Vac with 200 ml of
diluent at room temperature. Remove the centre tabs of aluminium
seals on both vaccine vial and diluent bottle, leaving intact the outer
ring on both vial and bottle. Cleanse rubber stoppers with alcohol and
allow to dry. All equipment used for vaccination should be sterile and
contain no traces of detergents or disinfectants. Using a sterile needle
and syringe, insert through diluent bottle stopper and withdraw 3 ml of
diluent. Transfer by inserting through vaccine vial stopper. After slight
agitation of the vial to ensure that the vaccine has dissolved, insert
needle and withdraw entire contents into the syringe. Remove the
syringe containing all the reconstituted vaccine from vial and re-insert
into diluent bottle. Expel syringe contents into diluent. The
reconstituted vaccine is now ready for use. For administration of the
vaccine an automatic syringe with a 23 gauge x 1 inch (0.60 x 25 mm)
needle is recommended. Inject each chick intramuscularly with 0.2 ml
of vaccine, the usual site being the upper thigh, or subcutaneously in
the neck.
Use within 2 hours of reconstitution.