Innovax-ND-IBD-ILT Concentrate and Solvent for Suspension for Injection for Chickens

Posology:
Subcutaneous use: one single injection of 0.2 ml per chick.
In ovo: one single injection of 0.05 ml per egg.
Preparation of the vaccine:
The usual aseptic precautions should be applied to all preparation and administration
procedures. The handling of liquid nitrogen should take place in a well-ventilated
area.
1. Use solvent for cell associated poultry vaccines for reconstitution. Reconstitute
the vaccine according to the tables below:
For subcutaneous use reconstitute the vaccine according to the table below:
Solvent bagNumber of vaccine ampoules for subcutaneous use
Bag of 400 ml solvent1 ampoule containing 2000 dosesBag of 800 ml solvent2 ampoules containing 2000 dosesBag of 800 ml solvent1 ampoule containing 4000 dosesBag of 1200 ml solvent3 ampoules containing 2000 dosesBag of 1600 ml solvent4 ampoules containing 2000 dosesBag of 1600 ml solvent2 ampoules containing 4000 doses

When this product is mixed with Nobilis Rismavac, both should be diluted in the
same solvent bag in the same way (400 ml of solvent for each 2000 doses of both
products or 800 ml of solvent for each 4000 doses of both products).
For in ovo use reconstitute the vaccine according to the table below:

Revised: April 2024
AN: 00493/2023
Solvent bagNumber of vaccine ampoules for in ovo useBag of 400 ml solvent4 ampoules containing 2000 dosesBag of 400 ml solvent2 ampoules containing 4000 dosesBag of 800 ml solvent8 ampoules containing 2000 dosesBag of 800 ml solvent4 ampoules containing 4000 dosesBag of 1200 ml solvent12 ampoules containing 2000 dosesBag of 1200 ml solvent6 ampoules containing 4000 dosesBag of 1600 ml solvent16 ampoules containing 2000 dosesBag of 1600 ml solvent 8 ampoules containing 4000 doses
The solvent must be clear, red coloured, without sediment and at room
temperature (15 °C –25 °C) at the time of mixing.
2. Preparation of the vaccine shall be planned before the ampoules are taken from
the liquid nitrogen and the exact amount of vaccine ampoules and amount of
solvent needed shall be calculated first. There is no information available on the
number of doses on the ampoules once they are removed from the cane, so
special care has to be taken to ensure that the mix-ups of ampoules with
different number of doses is avoided and the correct solvent is used.
3. Before withdrawing the ampoules from the liquid nitrogen container, protect
hands with gloves, wear long sleeves and use a facemask or goggles. When
removing an ampoule from the cane, hold in the palm of a gloved hand away
from the body and the face.
4. When withdrawing a cane of ampoules from the canister in the liquid nitrogen
container, expose only the ampoule(s) to be used immediately. It is
recommended to handle a maximum of 5 ampoules (from one cane only) at a
time. After removing the ampoule(s), the remaining ampoules should be put
back immediately into the canister in the liquid nitrogen container.
5. The content of the ampoule(s) is thawed rapidly by immersing in clean water at
25 °C –27 °C. Gently swirl the ampoule(s) to disperse the contents. It is
important that the ampoule content, after being thawed, is mixed immediately
into the solvent to protect the cells.
Dry the ampoule, then break the ampoule at its neck and immediately proceed
as described below.
6. Gently withdraw the contents of the ampoule into a sterile syringe, mounted with
an 18 gauge needle.
7. Insert the needle through the stopper of the solvent bag and add slowly and
gently the contents of the syringe to the solvent. Gently swirl and invert the bag
to mix the vaccine. Withdraw a portion of the solvent into the syringe to rinse the
ampoule. Remove the washing from the ampoule and inject it gently into the
solvent bag.
8. Repeat steps 6 and 7 for additional ampoules, if required.
9. Remove the syringe and invert the bag (6–8 times) to mix the vaccine
10. The vaccine is now ready for use.
After adding the content of the ampoule to the solvent, the ready to use product
is a clear, red coloured suspension for injection.
Administration:
The vaccine is administered by subcutaneous injection in the neck or by
injection. The bag of vaccine should be gently swirled frequently during vaccination to
guarantee that the vaccine suspension remains homogenous and that the correct
vaccine virus titre is administered (e.g. during long vaccination sessions).

Revised: April 2024
AN: 00493/2023
Control of correct storage:
To allow a check on correct storage and transport the ampoules are placed upside
down in the liquid nitrogen containers. If frozen content is situated in the tip of the
ampoule this indicates that the content has been thawed and must not be used.