Poulvac Marek CVI Concentrate and Solvent for Suspension for Injection

One 0.2 ml dose per chicken.
The vaccine is to be administered by intramuscular injection in the thigh
muscle or by subcutaneous injection in the neck.
Dilution of vaccine:
Reconstitute The 2,000 dose presentation with 400 ml of diluent,4000 dose
presentation with 800 ml of diluent and the 5,000 dose presentation with
1000 ml of diluent. Dilution should be done under sterile conditions with
diluent at room temperature. An ampoule may occasionally explode after it
has been taken out of liquid nitrogen so goggles should be worn.
Preparation of the vaccine shall be planned before the ampoules are taken
from the liquid nitrogen and the exact amount of vaccine ampoules and
amount of diluent needed shall be calculated first. There is no information
available on the number of doses on the ampoules once they are removed
from the cane. Special care has to be taken to ensure that the mix-ups of
ampoules with different number of doses is avoided and the correct diluent is
used.
Take the ampoule of vaccine out of the container of liquid nitrogen into a bowl
containing clean tepid water (temperature 27°C (80°F) and not higher than
37°C (98°F)). Thaw the vaccine concentrate by carefully turning the ampoule,
then remove from the water and dry the ampoule. The thawed vaccine
concentrate must be used immediately and cannot be refrozen.
All equipment used for vaccination should be sterile and contain no traces of
detergents or disinfectants.
Break the ampoule and withdraw the total contents carefully into a 10 ml sterile
disposable syringe, using an 18G x 1.5 inch (1.2 x 40 mm) or larger gauge
needle. Slowly withdraw about 8 ml of diluent into the syringe. Turn the
syringe 5-10 times to mix the contents well. Slowly transfer a small volume of
the mixture into the empty vaccine ampoule in order to remove the last
remnants of the vaccine and withdraw this small amount back into the syringe,

Revised: May 2025
AN: 00173/2025
and carefully transfer the entire contents of the syringe into the diluent bottle.
Rotate the bottle about 10 times to mix the contents well. The bottle of diluent
should be kept closed throughout the procedure.
The vaccine is now ready for use and should be used within 2 hours of
reconstitution.
Administration: Poulvac Marek CVI may be administered either manually,
preferably by using a multi-dose syringe dose and fitted with a 23G x 1 inch
(0.60 x 25 mm) needle, or by a vaccination machine.
Administer the vaccine intramuscularly into the thigh muscle or subcutaneously
in the neck.
NB During the vaccination procedure, rotate the bottle of reconstituted vaccine
solution every 5 minutes to prevent the cells from sedimenting.