Nobilis Rismavac + CA126 Concentrate and Solvent for Suspension for Injection for Chickens

Inject 0.2 ml reconstituted vaccine to each bird subcutaneously in the neck or
intramuscularly in the leg using a 20g x 1/2" needle with an approved repeating
syringe or automatic vaccinator.
Equipment used for vaccination should be sterile and contain no traces of detergents
or disinfectants.
In ovo vaccination:
In ovo use.
Inject one dose of the reconstituted vaccine to each 18-day embryonated egg with an
appropriate automatic in ovo vaccinator. The actual volume per dose may depend on
the settings of the in ovo vaccination equipment. This should not be less than 50 µl or
more than 100 µl. Depending on the volume to be administered the vaccine should be
reconstituted according to the instructions below. The egg should be in an upright
position with the blunt side up.
Reconstitution:
The actual volume of solvent per dose needed for reconstitution of the vaccine may
depend on the number of doses per ampoule, the route of administration and for in
ovo on the settings of the vaccination equipment. In the table below the volume of
solvent per ampoule for the various dose-presentations, routes of administration and
settings of the in ovo equipment are given.
AN: 00083/2025Page 4 of 6
Volume of solvent per ampoule
needed for reconstitution of the vaccine
Number of doses per
ampoule
sc / im
(0.2 ml per
dose)
in ovo
(0.05 ml per
dose)
in ovo
(0.1 ml per
dose)
1000 doses 200 ml50 ml100 ml2000 doses 400 ml100 ml200 ml4000 doses800 ml200 ml400 ml5000 doses1000 ml250 ml500 ml
After adding the contents of the ampoule to the solvent, the ready to use product is a
clear, red coloured suspension for injection.
Prior to reconstitution the vaccine is thawed. Great care should be taken - see 3.5
Special precautions for use. Remove one ampoule from the cane and immediately
replace the cane in the liquid nitrogen canister. Thaw the contents of the ampoule
rapidly by immersing in water at room temperature. Do not thaw in hot or ice-cold
water. Dry the ampoule and shake to disperse contents. After thawing open the
ampoule immediately and draw the entire contents into a sterile 5 - 10 ml syringe using
an 18 gauge needle to avoid rupturing the cells. Insert the needle through the stopper
of the solvent vial (which should be at room temperature) and draw up slowly a portion
of the solvent. Add the contents of the syringe to the remaining solvent. It is important
that this is done slowly, allowing the vaccine to run down the side of the bottle. Gently
shake the bottle as the vaccine is being mixed. Withdraw a portion of the vaccine and
use to rinse the ampoule. Inject the washing back in to the solvent vial. The
reconstituted vaccine must be handled gently and administered through wide gauge
needles to avoid rupturing the cells. Fill the sterilised repeating syringe/automatic
vaccinator according to the manufacturer’s instructions. The vial of reconstituted
vaccine should be kept in an ice bath when not being used.