PMSG Intervet 5000IU, Powder and Solvent for Solution for Injection

Reconstitute product immediately before use.
Reconstitute by dissolving the powder plug in about 5 ml of the solvent provided and
then syringe the resulting solution into the solvent vial to mix with the remaining
solvent to obtain a clear, colourless solution.
Cow:
In conjunction with the use of progestagen treatment when used for oestrus control
in non-cycling cattle:
400 - 700 IU.
Superovulation:
1500 - 4000 IU on day 9 - 13 of the oestrous cycle.
Sow:
Anoestrus post weaning (induction of oestrus is difficult until 40 days post-partum):
1000 IU by subcutaneous or intramuscular injection. Fertile oestrus usually follows in
3 - 7 days.
Ewe:
In conjunction with progestagen-releasing sponges when used out of the normal
breeding season:
500 IU by subcutaneous or intramuscular injection at the time of sponge removal.
Bitch:
Oestrus induction (subnormal oestrus with non-acceptance):
20 IU/kg by subcutaneous injection daily for 10 days, at day 10 an injection of 500 IU
hCG.
Superovulation in cattle
The product may be used for the superovulation of female donor cattle preparatory
to embryo transfer. As an example, the following regime has been successfully
applied: A single dose of the product (1500 - 4000 IU) is injected on day 9 - 13 of a
normal oestrous cycle (N.B. the exact dose of the product required to achieve
effective superovulation will depend upon a number of factors particularly the breed,
age, reproductive history, general health and nutritional status of the donor female
and will be subject to individual variation). Forty-eight hours after the injection,
luteolysis is induced by the injection of a prostaglandin analogue. Commonly one
and a half times the normal luteolytic dose is administered. Oestrus normally occurs
approximately 48 hours after the prostaglandin injection. Insemination is carried out
at 60 and 72 hours after prostaglandin injection.
Collection of fertilised embryos (flushing) is carried out 6-8 days after insemination,
suitable embryos being transferred to female recipient cattle whose oestrous cycles
have previously been synchronised with that of the donor female.
(Experience has shown that oestrous cycles in donor and recipient females should
be synchronised within ± 24 hours if reasonable success is to be expected). A further
prostaglandin treatment (commonly 1½ times the luteolytic dose) must be given at
the time of collection.
Note:
1. Despite the application of a suitable treatment regime certain individual donor
cows may fail to respond.
2. Wide variations in response may be expected between individual animals.
Repeated treatment of a single animal may also yield variable results.
3. The overall success of an embryo transfer exercise will inevitably be influenced
by the availability of suitable equipment and the skill and experience of the
operator.
Further information
PMSG has been used in cases of impaired spermatogenesis in male animals (horse
and bull 1000 - 3000 IU, boar and ram 500 - 750 IU, dog 400 - 800 IU, by
intramuscular injection twice weekly for 4-6 weeks), but its degree of efficacy in
these cases may be low.
PMSG is a protein hormone which acts on the ovary to stimulate the production of
follicles. The number of follicles produced can be influenced by the dose of PMSG
administered and this must be considered when, for instance, calculating the dose
for a particular flock of ewes in which oestrus synchronisation is desired. In general,
the further out of season that breeding is attempted and the lower the normal
prolificacy of the flock, the more PMSG that will be required.
An average dose of 500 IU/ewe is recommended as a useful starting point but doses
ranging from 200 - 750 IU have been used on occasion. It is therefore recommended
that accurate flock records are kept of breed, dose given, time of injection and lambs
produced so that in future seasons the amount can, if necessary, be adjusted for
optimum results.