Vectormune HVT-AIV Concentrate and Solvent for Suspension for Injection for Chickens

One single injection of 0.2 ml per chick. The vaccine may be injected by an automatic
syringe.
Table 1. Overview table for dilution possibilities of different presentations
Vaccine ampoule
presentation
(Number of vaccine
ampoules multiplied by
doses needed)
Solvent
presentation
(ml)
Volume of one
dose
(ml)
2 x 1,000 4001 x 2,000 4004 x 1,000 8002 x 2,000 8001 x 4,000 8004,000 + 1,000 1,0006 x 1,000 1,2003 x 2,000 1,2004 x 2,000 1,6002 x 4,000 1,600
0.20
The usual aseptic precautions should be applied to administration procedure.
Be familiar with all safety and precautionary measures for handling liquid nitrogen in order
to prevent personal injury.
Preparation of vaccine suspension for injection:
1. After matching the dose size of the vaccine ampoule presentation with the solvent bag
size, quickly remove the exact number of ampoules needed from the liquid nitrogen
container.
2. Draw up 2 ml of solvent into a 5 ml syringe.
3. Thaw rapidly the contents of the ampoules by gentle agitation in water at 27–39 °C.
4. As soon as they are completely thawed, open ampoules holding them at arm’s length in
order to prevent any risk of injury should the ampoule break.
5. Once the ampoule is open, slowly draw up the content into the 5 ml sterile syringe
already containing 2 ml of solvent with a needle of at least 18-gauge diameter.
6. Transfer the suspension into the solvent bag. The diluted vaccine prepared as
described is mixed by gentle agitation.
7. Withdraw a portion of the diluted vaccine into the syringe to rinse the ampoule. Remove
the washing from the ampoule and inject it gently into the solvent bag. Repeat one or
two times.
8. The diluted vaccine prepared as described is mixed by gentle agitation so as to be
ready for use.
9. It should be ensured that the vaccine suspension is mixed regularly in a gentle way
during the vaccination session to guarantee that the vaccine suspension remains
homogenous.
Repeat the operations in point 2 to 7 for the appropriate number of ampoules to be
thawed.
Use the vaccine immediately, slowly mix regularly to ensure uniform suspension of cells
and use within a period not exceeding 2 hours.
The reconstituted vaccine is a clear, red-coloured suspension for injection.
Discard any ampoules that have been accidentally thawed.
Do not re-freeze under any circumstances.
Do not re-use open containers of reconstituted vaccine.