Poulvac Procerta HVT-IBD Concentrate and Solvent for Suspension for Injection for Chickens

One single injection of 0.2 ml per chicken at day of hatch, by subcutaneous use.
One single injection of 0.05 ml per chicken egg at 18-19 days of embryonation, by in
ovo route.
Preparation of the vaccine:
Preparation of the vaccine shall be planned before the ampoules are taken from the
liquid nitrogen and the exact amount of vaccine ampoules and amount of solvent
needed shall be calculated first. There is no information available on the number of
doses on the ampoules once they are removed from the cane. Special care has to
be taken to ensure that mix-ups of ampoules with different number of doses is
avoided and the correct volume of solvent (Poulvac Solvent) is used.
For subcutaneous use, reconstitute each 2,000 doses with 400 ml of Poulvac
Solvent and each 4,000 doses with 800 ml of Poulvac Solvent. For in ovo use,
reconstitute each 2,000 doses with 100 ml of Poulvac Solvent and 4,000 doses with
200 ml of Poulvac Solvent. The solvent must be at room temperature (15 °C – 25 °C)
at the time of mixing with the vaccine.
Overview tables for the dilution examples for the different dose presentations for
both subcutaneous and in ovo administration are provided:
Poulvac Solvent bag Number of vaccine ampoules for
subcutaneous use
Bag of 400 ml solvent 1 ampoule containing 2,000 doses Bag of 800 ml solvent 2 ampoules containing 2,000 doses Bag of 800 ml solvent 1 ampoule containing 4,000 doses Poulvac Solvent bag Number of vaccine ampoules for in ovo use Bag of 200 ml solvent2 ampoules containing 2,000 dosesBag of 400 ml solvent 4 ampoules containing 2,000 doses Bag of 400 ml solvent2 ampoules containing 4,000 doses Bag of 800 ml solvent 4 ampoules containing 4,000 doses Bag of 1,000 ml solvent5 ampoules containing 4,000 doses
Reconstitution should be done under aseptic conditions. Before withdrawing the
ampoules from the liquid nitrogen container, protect the hands with gloves, wear long
sleeves and use a face shield or goggles.
It is recommended to handle a maximum of 5 ampoules at a time. After removing the
ampoule(s), the remaining ampoules should be put back immediately into the
canister in the liquid nitrogen container.
Take the ampoule(s) of vaccine out of the liquid nitrogen container and thaw the
vaccine by immersing in water at 25 ºC – 30 ºC, while gently swirling the ampoule(s)
to disperse the content. As soon as vaccine in the ampoule is completely thawed,
remove from the water, dry the ampoule and break the ampoules at its neck.
Once opened, slowly withdraw the total contents of the ampoule carefully into a
10 ml sterile disposable syringe with an 18-gauge needle. Slowly draw about 8 ml of
Poulvac Solvent into the syringe. Turn the syringe 5-10 times to mix the contents
well. Slowly transfer a small volume of the mixture into the empty vaccine ampoule in
order to rinse the ampoule and withdraw this small amount back into the syringe.
Carefully transfer the entire content of the syringe into the Poulvac Solvent container.
Remove the syringe and invert the solvent bag about 10 times to mix the vaccine.
The vaccine is now ready for use.
The ready to use vaccine is a red, slightly opalescent liquid.
In case automated equipment is used for in ovo or subcutaneous administration, the
equipment should be calibrated to ensure that the correct dose is applied to each
egg or chicken. The instructions for use of this device should be followed.
The bag of vaccine should be gently swirled frequently during vaccination to
guarantee that the vaccine suspension remains homogenous and that the correct
vaccine virus titre is administered.