ULTIFEND ND IBD Concentrate and Solvent for Suspension for Injection for Chickens

In ovo and subcutaneous use.
In ovo administration: one dose of 0.05 ml to be administered to 18-day-old chicken
embryonated eggs.
Subcutaneous administration: one dose of 0.2 ml to be administered to one-day-old
chicken, in the skin of the neck.
Preparation of vaccine:
Use sterile devices and equipment for reconstitution and for administration of the
vaccine. Before withdrawing vaccine from liquid nitrogen container, protect hands
with gloves and use goggles and boots. When removing an ampoule from the strip,
hold palm of gloved hand away from body and face.
1. After matching the dose size of the vaccine with the solvent size, quickly remove
from liquid nitrogen container the exact number of ampoules needed.
2. Draw up 2 to 5 ml of solvent into a 5 to 10 ml sterile syringe. Use at least 18-gauge
needles.
3. Thaw rapidly the contents of the ampoules by gentle agitation in water at 27-39 °C.
4. As soon as they are completely thawed, open ampoules holding them at arm’s
length in order to prevent any risk of injury should the ampoule break.
5. Once the ampoule is open, slowly draw up the content into the needle already
containing 2 to 5 ml solvent.

Revised: October 2022
AN: 01733/2022
6. Transfer the suspension into the solvent bag. The diluted vaccine prepared as
described is mixed by gentle agitation.
7. Withdraw a portion of the diluted vaccine into the syringe to rinse ampoule.
Remove the washing from the ampoule and inject it gently into the solvent bag.
Repeat it one or two times.
8. The diluted vaccine prepared as described is mixed by gentle agitation so as to be
ready for use.
Repeat the operations in point 2 to7 for the appropriate number of ampoules to be
thawed.
The ready to use vaccine is a red, slightly opalescent liquid.
Proposed dilutions for in-ovo administration:
One single dose of 0.05 ml is injected into each 18-day-old chicken embryonated
egg.
Number of vaccine
vials
Solvent Volume of one
dose
4 x 2000 doses400 ml0.05 ml2 x 4000 doses400 ml0.05 ml4 x 4000 doses800 ml0.05 ml5 x 4000 doses1000 ml0.05 ml6 x 4000 doses1200 ml0.05 ml8 x 4000 doses1600 ml0.05 ml
Proposed dilutions for subcutaneous administration:
One single injection of 0.2 ml per chick is applied at one day of age.
Number of vaccine
vials
Solvent Volume of one
dose
2 x 1000 doses400 ml0.2 ml1 x 2000 doses400 ml0.2 ml1 x 4000 doses800 ml0.2 ml3 x 2000 doses1200 ml0.2 ml2 x 4000 doses1600 ml0.2 ml