HuveGuard NB Suspension for Oral Suspension for Chickens

In drinking water: administer one dose of vaccine to each chicken from 3 days of age.
Once the 30 ml containing 1000 or 5000 doses vial is opened, the entire contents
must be used.
Administration via spray onto feed
Sufficient starter feed for the chicks’ first 12-24 hours should be laid out on paper or
plastic along the floor of the poultry house.
Shake the vaccine vial vigorously for 30 seconds before use to re-suspend the
oocysts. Dilute the vaccine in water at the rate of approximately 1000 doses in 1 litre
of water (5000 doses in 5 litres). To ensure that all oocysts are removed from the vial,
rinse it out 3 times with water. Spray the oocyst suspension evenly over the surface
of the feed using a coarse spray. Ensure a controlled even coverage of the total
surface area of the feed available to the chickens. Agitate the applicator reservoir
regularly throughout spraying to avoid settling-out of oocysts. Ensure that all
available feed is treated and that the total number of doses dispensed matches the
number of birds in the house.
Once the vaccine has been diluted for use it should be sprayed immediately onto
feed and birds should be placed with access to the feed immediately.
When the treated allocation of feed has been consumed, routine feeding may
continue.
It is recommended to monitor the feed intake and behaviour of the birds and to apply
the vaccine by this method only after an adequate feed intake is expected.
AN: 00937/2025 & 01785/2025
Administration via drinking water
For the administration of the vaccine drinkers must be used.
Provide an adequate number of drinkers and drinking space so that all chicks have
access to the vaccinal water and thus can receive the correct dose.
Place the drinkers evenly in the area where chicks are housed.
Water should be withheld for 2–4 hours before vaccination.
Preparation of the xanthan gum solution:
Commercially available xanthan gum can be used.
For 1000 doses put 3 litres of clean drinking water at room temperature in a suitable
container and dissolve 5 g xanthan gum.
For 5000 doses put 15 litres of clean drinking water at room temperature in a suitable
container and dissolve 25 g xanthan gum.
Prepare the vaccine suspension as follows:
To resuspend the oocysts, shake the vaccine vial vigorously. Open the vial and pour
the whole contents into clean drinking water at room temperature: 2 litres for 1000
doses and 10 litres for 5000 doses. To ensure that all oocysts are removed from the
vial, rinse it out 3 times with water. Shake the obtained 2 litres (1000 doses) or 10
litres (5000 doses) of vaccine suspension and transfer gradually into the prepared
xanthan gum solution, mixing thoroughly to ensure a homogeneous suspension.
Mixing the xanthan gum solution together with the vaccine suspension will result in a
final quantity of 5 litre (for 1000 doses) or 25 litre (for 5000 doses) vaccine-xanthan
gum suspension. Pour the vaccine-xanthan gum suspension into the drinking
equipment.
Administration via spray on chickens.
For each 100 birds a dose volume of about 24 ml (0.24 ml/bird) of coarse spray
suspension has to be prepared.
For spraying on chickens use Brilliant Blue (E133) coloring agent.
Preparation of the coloured diluent:
For 1000 doses put 240 ml of water in a suitable container and add Brilliant Blue
(E133) colorant at a concentration of 0.01% w/v.
For 5000 doses put 1200 ml of water in a suitable container and add Brilliant Blue
(E133) colorant at a concentration of 0.01% w/v.
Preparation and administration of the vaccine suspension:
Shake the 1000- or 5000- doses vial vigorously to resuspend the oocysts.
Add the entire content of the vial to the diluent and mix thoroughly. Rinse the vial 3
times with diluent to ensure that all oocysts are removed. Fill the vaccine reservoir of
the spraying device with the full volume prepared. Continuously maintain
homogeneity of the vaccine suspension. The pressure of the spraying device should
be at 3 bars. The spraying device must provide a droplet size of ≥100 µm.
To improve the uniformity of the vaccination, maintain the chicks inside the chick box
for at least 1 hour in order to let them ingest all the vaccine droplets. Make sure that
there is enough light so that the chickens are awake and preen themselves and each
other.
AN: 00937/2025 & 01785/2025